粪便DNA提取试剂盒 FastDNA Spin Kit For Feces
货号：116570200 品牌 MP Biomedicals
快速高效提取粪便DNA，可裂解任何粪便样品。可直接用于PCR等其下游实验，The FastDNA® SPIN Kit for Feces Designed for use with the FastPrep® Instruments, host cells as well as bacteria, fungi, viruses, protists and other cells present in fecal samples are easily lysed within 40 seconds. These benchtop devices use a unique, optimized motion that provide an extremely quick, efficient and highly reproducible homogenization that surpasses traditional extraction methods.
Samples are placed into 2.0 mL tubes containing Lysing Matrix E, a mixture of ceramic and silica particles designed to efficiently lyse all stool organisms. Stool samples are pre-treated by washing in a Pre-Lysis Buffer. Homogenization takes place in the presence of MT Buffer and Sodium Phosphate Buffer. The pre-lysing and lysing reagents were carefully developed to protect and solubilize nucleic acids upon cell lysis, facilitate extraction of genomic DNA , and minimize RNA, humic acid or polyphenol contamination. Following lysis, samples are centrifuged to pellet debris and lysing matrix. DNA is purified from the supernatant with a silica-based GENECLEAN® procedure using SPIN filters. Eluted DNA is ready for digestion, electrophoresis, PCR and any other desired application.
The Fast DNA® SPIN Kit for Feces quickly and efficiently isolates PCR-ready genomic DNA from fresh or frozen human and animal stool samples.
Product Type Kits & Assays
Components Lysing Matrix E 50x 2.0 mL tubes, PLS Solution 25 mL, MT Buffer 8 mL, Sodium Phosphate Buffer 100 mL, PPS 25 mL, Binding Matrix 66 mL, Wash Buffer #1 120 mL, Wash Buffer #2 12 mL, TES 20 mL, BBS Gel Loading Dye 200 μl, SPIN Filter Tubes 50 each, Catch Tubes 50 each
Instrument Compatibility FastPrep-24; FastPrep 120; FastPrep-96; SuperFastPrep-1
Sample Type Soil; Feces; Environmental Water; Wastewater; Sludge
Sample Volume up to 500 mg per prep
Tube Compatibility 2 mL
Matrix Type E
Molecule Enriched DNA
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